Transformation of Escherichia coli with a large plasmid of Acidiphilium multivorum AIU 301 encoding arsenic resistance.

نویسندگان

  • K Suzuki
  • N Wakao
  • Y Sakurai
  • T Kimura
  • K Sakka
  • K Ohmiya
چکیده

Acidiphilium multivorum AIU 301 isolated from acid mineral water had strong arsenic resistance. This bacterium harbored a number of plasmids with different molecular sizes. A plasmid of 56 kbp, named pKW301, was isolated from A. multivorum AIU 301. When pKW301 was transferred into Escherichia coli JM109 by electroporation, an E. coli transformant carrying pKW301 exhibited resistance to sodium arsenite, sodium arsenate, and mercuric (II) chloride.

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Expression and regulation of the arsenic resistance operon of Acidiphilium multivorum AIU 301 plasmid pKW301 in Escherichia coli.

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عنوان ژورنال:
  • Applied and environmental microbiology

دوره 63 5  شماره 

صفحات  -

تاریخ انتشار 1997